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human il 21 standard (Sino Biological)

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Sino Biological human il 21 standard
Human Il 21 Standard, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il+21+standard/Human+Interleukin-21+%2F+IL-21+Protein/us12508309-155-1-5
Average 94 stars, based on 21 article reviews

human il 21 standard - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Recombinant viral vector, immunogenic composition comprising same, and uses
Article Snippet: Mino cells (ATCC® CRL-3000) were cultured in a complete RPMI-1640 medium (10% fetal bovine serum (FBS), 1% penicillin-streptomycin (PS)) to a log phase, and 125 g of culture was centrifuged to collect the cells, and diluted to 2×105/mL by the complete medium. .. A human IL-21 standard (Beijing Sino Biological, Cat. No.: 10584-HNAE) was diluted stepwise in a 96-well plate by a two-fold gradient, and used as blank controls, a total of 12 gradients, 100 microliters per well; after the dilution was completed, 50 microliters of uniformly mixed cells (1×104 cells) were added, a total of 150 microliters of liquid per well; and the 96-well plate was placed and cultured in a 37° C., 5% CO2 incubator for 6-7 days. .. 10 microliters of CCK-8 reagent (MCE, Cat. No.: HY-K0301) was added to each well of the 96-well plate after the culture, the 96-well plate was put back into the incubator for a continued culture of 4-8 hours (the time was determined according to color change), and OD450 was measured with a microplate reader.

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